Welcome to the Homepage of
Andy Vierstraete

I'm working under the all seeing eye of Prof. Dr. Bart Braeckman who is working on ageing of Caenorhabditis elegans , globines and phylogeny of nematodes, part of the Department of Biology, Faculty of Science, Ghent University

• The central dogma of molecular biology
• The mystery of sequencing for phylogenetic research...
  1. What is DNA ?
  2. DNA in a cell
  3. Preparation of gDNA
  4. PCR to amplify the requested gene
  5. Sequencing of the obtained PCR product
  6. Aligning of the gene with genes from other species
  7. Calculating the phylogenetic trees

• Next Generation Sequencing

• Phylogenetic trees of nematodes

• Protocols we use
• Fixation of the organism for DNA research
• Sucrose wash of cultured nematodes
• gDNA extraction
• RNA extraction
• Cloning
• Sequencing on ABI 3130XL Genetic Analizer
• Tricks we use...
• Who the heck am I ?
• What's still under construction for my webpages ?
• What's new on these pages since the origin of life ?
(new stuff since 20-05-2012)
• Links to other pages
• Drawings and principles in Acrobat (.pdf) format
• Drawings in .tif format (600 dpi)
• A little program I have written : Cutoff
• The elision alignment from the publication : Phylogenetic Relationships within the Cyst-Forming Nematodes (Nematoda, Heteroderidae) Based on Analysis of Sequences from the ITS Regions of Ribosomal DNA.
• Veiligheidsetiketten voor recipienten met gevaarlijke en/of brandbare produkten (internal use)