Department of Biology

Ghent University




46. Claeys, M., Yushin, V.V., Leunissen, J. L.M., Claeys, J. and Bert, W.  Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes. Nematology 19(8), 871-881. (2017).


45. Yushin, V.V., Subbotin, S.A., Claeys, M. and Bert, W. Spermatozoa in spermatheca of the plant-parasitic nematode Paratylenchus hamatus sensu lato (Tylenchida, Paratylenchidae). Russian Journal of Nematology 25 (2), 93-99 (2017).


44. Raaymakers, C., Verbrugghe, E., Hernot, S., Hellebuyck, T., Betti, C., Peleman, C., Claeys, M., Bert, W., Caveliers, V., Ballet, S., Martel, A., Pasmans, F. & Roelants, K. (2017). Antimicrobial peptides in frog poisons constitute a molecular toxin delivery system against predators. Nature Communications. In press.


43. De Swaef, E., Claeys, M., Bert, W.,  Huysseune, A., Witten, P.,  Van Den Broeck, W.,  and Decostere, A.  (2017).

Ultrastructural morphology of the envelope of Dover sole Solea solea eggs from fertilization until hatching with emphasis on sample preparation. Micron 99. p.9-18.


42. Xue, Q., Decraemer, W., Claeys, M.,  and Bert, W.  (2017).  Molecular phylogeny of Malenchus and Filenchus (Nematoda: Tylenchidae).  Zoolocica Scripta. DOI: 10.111/zsc. IF 2015 2.733; Q1 Aoology 12/161.


41. Xue, Q., Slos, D., Claeys, M. and Bert, W. (2017). Ultrastructural, phylogenetic and rRNA secondary structural analysis of a new nematode (Nematoda: Tylenchomorpha) from mushroom with recovery of intestinal crystals. Zoologischer Anzeiger. 269. p.13-25. [IF 2016 1.2; Q2 Zoology 69/162].


40. Yushin, V. V., Claeys, M. & Bert, W. (2016).Ultrastructural immunogold localization of major sperm protein (MSP) in spermatogenic cells of the nematode Acrobeles complexus (Nematoda, Rhabditida). Micron 89, 43-55, 43-55.


39. Van Wichelen, J., D’hondt, S., Claeys, M., Vyverman, W., Berney, C., Bass, D. & Vanormeling, P. (2016). A hotspot of amoebae diversity: 8 new naked amoebae associated with the planktonic bloom-forming cyanobacterium Microcystis. Acta Protozoologica 51


38. Bert, W., Slos, D., Leroux, O. & Claeys, M. Cryo-fixation and associated developments in transmission electron microscopy: a cool future for nematology. Nematology 18, 1-14, doi:10.1163/15685411-00002943 (2016).


37. Slos, D., Ensafi, P., Claeys, M., Yushin, V. V., Decraemer, W. & Bert, W. Ultrastructure of sperm development in the genus Ditylenchus (Nematoda: Anguinidae). Nematology 17, 313-324 (2015).


36. Lambrecht, E., Baré, J., Claeys, M., Chavatte, N., Bert, W., Sabbe, K. & Houf, K. Transmission electron microscopy sample preparation protocols for ultrastructural study of cysts of free-living protozoa. Biotechniques 58, 181-188, doi: 10.2144/000114274 (2015).


35. Lak, B., Yushin, V. V., Slos, D., Claeys, M., Decraemer, W. & Bert, W. High-pressure freezing and freeze-substitution fixation reveal the ultrastructure of immature and mature spermatozoa of the plant-parasitic nematode Trichodorus similis (Nematoda; Triplonchida; Trichodoridae). Micron 77, 25-31, doi:10.1016/j.micron.2015.05.012 (2015).


34. Van Waeyenberghe, L., Bare, J., Pasmans, F., Claeys, M., Bert, W., Haesebrouck, F., Houf, K. & Martel, A. Interaction of Aspergillus fumigatus conidia with Acanthamoeba castellanii parallels macrophage-fungus interactions. Env Microbiol Rep 5, 819-824 (2013).


33. Yushin, V. V., Claeys, M. & Houthoofd, W. Mature spermatozoa of Brevibucca sp (Nematoda: Rhabditida: Brevibuccidae). Russian Journal of Nematology 19, 131-138 (2011).


32. Leroux, O., Leroux, F., Bagniewska-Zadworna, A., Knox, J. P., Claeys, M., Bals, S. & Viane, R. L. L. Ultrastructure and composition of cell wall appositions in the roots of Asplenium (Polypodiales). Micron 42, 863-870, doi:10.1016/j.micron.2011.06.002 (2011).


31. Leroux, O., Knox, J. P., Masschaele, B., Bagniewska-Zadworna, A., Marcus, S. E., Claeys, M., van Hoorebeke, L. & Viane, R. L. L. An extensin-rich matrix lines the carinal canals in Equisetum ramosissimum, which may function as water-conducting channels. Annals of Botany 108, 307-319, doi:10.1093/aob/mcr161 (2011).


30. Leroux, O., Bagniewska-Zadworna, A., Rambe, S. K., Knox, J. P., Marcus, S. E., Bellefroid, E., Stubbe, D., Chabbert, B., Habrant, A., Claeys, M. & Viane, R. L. L. Non-lignified helical cell wall thickenings in root cortical cells of Aspleniaceae (Polypodiales): histology and taxonomical significance. Annals of Botany 107, 195-207, doi:10.1093/aob/mcq225 (2011).


29. Boone, M., Bert, W., Claeys, M., Houthoofd, W. & Artois, T. Spermatogenesis and the structure of the testes in Nemertodermatida. Zoomorphology 130, 273-282, doi:10.1007/s00435-011-0137-9 (2011).


28. Haegeman, Annelies, B. V., Jacob, J., Vandekerckhove, T. T. M., Claeys, M., Borgonie, G. & Gheysen, G. A Wolbachia-

like endosymbiotic bacterium in the plant-parasitc nematode Rhadopholus similis. Journal of Nematology 42, 245-245 (2010).


27. Willems, M., Leroux, F., Claeys, M., Boone, M., Mouton, S., Artois, T. & Borgonie, G. Ontogeny of the Complex Sperm in the Macrostomid Flatworm Macrostomum lignano (Macrostomorpha, Rhabditophora). Journal of Morphology 270, 162-174 (2009).


26. Leroux, O., Leroux, F., Bellefroid, E., Claeys, M., Couvreur, M., Borgonie, G., Van Hoorebeke, L., Masschaele, B. & Viane, R. A new preparation method to study fresh plant structures with X-ray computed tomography. Journal of Microscopy-Oxford 233, 1-4, doi:10.1111/j.1365-2818.2008.03088.x (2009).


25. Fonderie, P., Willems, M., Bert, W., Houthoofd, W., Steel, H., Claeys, M. & Borgonie, G. Intestine ultrastructure of the facultative parasite Halicephalobus gingivalis (Nematoda: Panagrolaimidae). Nematology 11, 859-868 (2009).


24. Bert, W., Claeys, M. & Borgonie, G. The comparative cellular architecture of the female gonoduct among Tylenchoidea (Nematoda : Tylenchina). Journal of Nematology 38, 362-375 (2006).


23. Willems, M., Houthoofd, W., Claeys, M., Couvreur, M., Van Driessche, R., Adriaens, D., Jacobsen, K. & Borgonie, G. Unusual intestinal lamellae in the nematode Rhabditophanes sp KR3021 (Nematoda : Alloinematidae). Journal of Morphology 264, 223-232 (2005).


22. Van Gansbeke, R., Bert, W., Govaerts, A., Claeys, M., Van Driessche, R., Manhout, J. & Borgonie, G. Cuticular structure of three Bunonema species and first description of the male of B. penardi Stefanski, 1914 (Nematoda : Bunonematidae). Nematology 6, 473-484 (2004).


21. Tytgat, T., Vanholme, B., De Meutter, J., Claeys, M., Couvreur, M., Vanhoutte, I., Gheysen, G., Van Criekinge, W., Borgonie, G. & Coomans, A. A new class of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in plant parasitic cyst nematodes. Molecular Plant-Microbe Interactions 17, 846-852 (2004).


20. Claeys, M., Vanhecke, D., Couvreur, M., Tytgat, T., Coomans, A. & Borgonie, G. High-pressure freezing and freeze substitution of gravid Caenorhabditis elegans (Nematoda : Rhabditida) for transmission electron microscopy. Nematology 6, 319-327, doi:10.1163/1568541042360564 (2004).


19.  Bossinger, O., Fukushige, T., Claeys, M., Borgonie, G. & McGhee, J. D. The apical disposition of the Caenorhabditis elegans intestinal terminal web is maintained by LET-413. Developmental Biology 268, 448-456 (2004).


18.  Borgonie, G., Link, C. D., Claeys, M. & Coomans, A. Lysosomal and pseudocoelom routing protects Caenorhabditis elegans from ricin toxicity. Nematology 5, 339-350 (2003).


17. Bert, W., Van Gansbeke, R., Claeys, M., Geraert, E. & Borgonie, G. Comparative morpho-anatomical studies of the female gonoduct within the Pratylenchidae (Nematoda: Tylenchina). Nematology 5, 293-306 (2003).


16. Tytgat, T., De Meutter, J., Vanholme, B., Claeys M., Verreijdt, L., Gheysen, G. & Coomans, A. (2002). Development and pharyngeal gland activities of Heterodera schachtii infecting Arabidopsis thaliana roots. Nematology 4, 899-908.


15. Coomans, A., Vandekerckhove, T.T.M. & Claeys, M. (2000). Transovarial transmission of symbionts in Xiphinema brevicollum (Nematoda: Longidoridae). Nematology 2, 443-449.


14. Vancompenolle, B., CLaeys, M., Borgonie, G., Tytgat, T. & Coomans, A. (2000). Evaluation of fixation methods for ultrastructural study of Caenorhabditis elegans embryos. Microscopy Research and Technique 49, 212-216.


13. Vandekerckhove, T.T.M., Willems, A., Claeys, M., Gillis, M. & Coomans, A. (2000). Occurrence of novel verrucomicrobial species, endosymbiotic in Xiphinema americanum-group species (Nematoda, Longidoridae) and associated with  parthenogenesis. International Journal of Systematic and Evolutionary Microbiology 50, 2197-2205.


12. Coomans, A. & Claeys, M. (1998). Structure of the female reproductive system of Xiphinema americanum (Nematoda: Longidoridae). Fundam. appl. Nematol. 21, 569-580.


11. Borgonie, G., Van Driessche, E., Link, C.D., CLaeys, M., De Waele, D. & Coomans, A. (1997). Internal lectin binding patterns in the nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus. Fundam. appl. Nematol. 20, 173-186.


10. Borgonie, G., Claeys, M., Leyns, F., Arnaut, G., De Waele, D. & Coomans, A. (1996a). Effect of nematicidal Bacillus thuringiensis strains on free-living nematodes.  1. Light microscopic observations, species and biological stage specificity andidentification of restistant mutants of Caenorhabditis elegans. Fundam. appl. Nematol. 19, 391-398.


9. Borgonie, G., Claeys, M., Leyns, F., Arnaut, G., De Waele, D., Coomans, A. (1996b). Effect of nematicidal Bacillus thuringiensis strains on free-living nematodes.  2. Ultrastructural analysis of the intoxication process in Caenorhabditis elegans. Fundam. appl. Nematol. 19, 407-414.


8. Borgonie, G., Claeys, M., Leyns, F., Arnaut, G., De Waele, D. & Coomans, A. (1996c). Effect of a nematicidal Bacillus thuringiensis strain on free-living nematodes.  3. Characterization of the intoxication process. Fundam. appl. Nematol. 19, 523-528.


7. Borgonie, G., Claeys, M., De Waele, D. & Coomans, A. (1995a). In vivo and in vitro characterization of the intestine of fifteen bacteriophagous nematodes (Nematoda: Rhabditida). Fundam. appl. Nematol. 18, 115-122.


6. Borgonie, G., Claeys, M., De Waele, D. & Coomans, A. (1995b). Ultrastructure of the intestine of the bacteriophagous nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus (Nematoda: Rhabditida). Fundam. appl. Nematol. 18, 123-133.


5. Borgonie, G., Claeys.,  M., Vanfleteren, J., De Waele, D. & Coomans, A. (1995). Presence of peritrophic-like membranes in the intestine of three  bacteriophagous nematodes (Nematoda: Rhabditida). Fundam. appl. Nematol. 18, 227-233.


4. Coomans, A., CLaeys M. & Heyns, J. (1992). Ultrastructure of the uterus of Xiphinema pinoides (Nematoda). Fundam. appl. Nematol. 15, 341-346.


3. Van De Velde, M.C., Coomans, A., Van Ranst, L., Kruger J.C. & Claeys, M. (1991). Ultrastructure of Sperm Cells in the Female Gonoduct of Xiphinema. Tissue & Cell 23, 881-891.


2. Van De Velde, M., Coomans A., Heyns, J. & Claeys, M. (1990). Ultrastructure of the female reproductive system of Xiphinema  meridianum (Nematoda).  Revue Nématol. 13, 211-223.


1. Van De Velde, M., Coomans, A., Heyns, J., Claeys, M. & Hutsebaut, M. (1990). Ultrastructure of the female gonoduct of Xiphinema theresiae (Nematoda). Revue Nématol. 13, 449-461.






Other publication of the Nematology TEM facility (no co-authorship)


15. Stegen, G.,  Pasmans, F.,  Schmidt, B.R.,  Rouffaer, L., Van Praet, S., Schaub,M.,  Canessa, S., Laudelout, A.,  Kinet, T.,  Adriaensen, C., Haesebrouck, F., Bert, W.,  Bossuyt, F. and Martel, A. Drivers of salamander extirpation mediated by Batrachochytrium salamandrivorans. Nature. 544(7650). p.353-356. (2017).


14. Braeckman, B.P. & D’hondt I. Lifespan extension in Caenorhabditis elegans insulin/IGF-1 signalling mutants is supported by non-vertebrate physiological traits. Nematology (19) 499-508 (Forum Article). (2017).


13. Slos, D.,  Sudhaus, W.,  Stevens, L., Bert, W. and Blaxter; M. Caenorhabditis monodelphis sp. n. : defining the stem morphology and genomics of the genus Caenorhabditis. (2017). BMC Zoology.


12. Van Vaerenbergh, B.,  Lauwaert, J., Bert , W., Thybaut, J., De Clercq, J. and Vermeir, P.(2017) Effect of the ion exchange resin functionality on the catalytic activity and leaching of palladium nanoparticles in Suzuki cross-coupling. Chemcatchem. 9(3). p.451-457


11. Verbrugghe, E., Dhaenens, M., Leyman, B., Boyen, F., Shearer, N., Van Parys, A., Haesendonck, R., Bert, W., Favoreel, H., Deforce, D., Thompson, A., Haesebrouck, F. & Pasmans, F. Host stress drives Salmonella recrudescence. Scientific Reports 6, 20849 DOI: 20810.21038/srep20849 (2016).


10. Yu, T. S., Witten, P. E., Huysseune, A., Buettner, A., To, T. T. & Winkler, C. Live imaging of osteoclast inhibition by bisphosphonates in a medaka osteoporosis model. Disease Models & Mechanisms 9, 155-163, doi:10.1242/dmm.019091 (2016).


9. Dhondt, I., Vladislav, A. P., Huaihan, C., Vierstraete, A., Smith, R. D., Depuydt, G. & Braeckman, B. P. FOXO/DAF-16 activation slows down turnover of the majority of proteins in C. elegans. Cell Reports, in press (2016).


8. Lambrecht, E., Baré, J., Chavatte, N., Bert, W., Sabbe, K. & Houf, K. Protozoan cysts act as survival niche and protective shelter for foodborne pathogenic bacteria. Applied and Environmental Microbiology (2015).


7. De Henau, S., Tilleman, L., Vangheel, M., Luyckx, E., Trashin, S., Pauwels, M., Germani, F., Vlaeminck, C., Vanfleteren, J. R., Bert, W., Pesce, A., Nardini, M., Bolognesi, M., De Wael, K., Moens, L., Dewilde, S. & Braeckman, B. P. A redox signalling globin is essential for reproduction in Caenorhabditis elegans. Nature Communications 6, doi:10.1038/ncomms9782 (2015).


6. Martel, A., Spitzen-van der Sluijs, A., Blooi, M., Bert, W., Ducatelle, R., Fisher, M. C., Woeltjes, A., Bosman, W., Chiers, K., Bossuyt, F. & Pasmans, F. Batrachochytrium salamandrivorans sp nov causes lethal chytridiomycosis in amphibians. P Natl Acad Sci USA 110, 15325-15329, doi:10.1073/pnas.1307356110 (2013).


5. Lambrecht, E., Bare, J., Van Damme, I., Bert, W., Sabbe, K. & Houf, K. Behavior of Yersinia enterocolitica in the presence of the bacterivorous Acanthamoeba castellanii. Applied and Environmental Microbiology 79, 6407-6413, doi:10.1128/aem.01915-13 (2013).


4. Leroux, O., Knox, J. P., Leroux, F., Vrijdaghs, A., Bellefroid, E., Borgonie, G. & Viane, R. L. L. Intercellular pectic protuberances in Asplenium: New data on their composition and origin. Annals of Botany 100, 1165-1173, doi:10.1093/aob/mcm210 (2007).


3. Yushin, V. V., Coomans, A., Borgonie, G. & Malakhov, V. V. Ultrastructural study of the tadpole stage of the primitive marine nematode Enoplus demani (Enoplia : Enoplida). Russian Journal of Nematology 10, 43-53 (2002).


2. Yushin, V. V., Coomans, A., Borgonie, G. & Malakhov, V. V. Ultrastructural study of cuticle formation during embryogenesis of the free-living marine nematode Enoplus demani (Enoplida). Invertebrate Reproduction & Development 42, 189-203 (2002).


1. Borgonie, G., Vandriessche, E., Link, C. D., Dewaele, D. & Coomans, A. TISSUE TREATMENT FOR WHOLE-MOUNT INTERNAL LECTIN STAINING IN THE NEMATODES CAENORHABDITIS-ELEGANS, PANAGROLAIMUS-SUPERBUS AND ACROBELOIDES-MAXIMUS. Histochemistry 101, 379-384, doi:10.1007/bf00269000 (1994).






4 th  International Congress on Invertebrate Morphology, Moscow, 18 – 23 August 2017.

Claeys, M., Yushin, V.V., Leunissen, J., Claeys, J. and Bert, W. Self-Pressurized rapid freezing (SPRF): An easy to use and low-cost alternative cryo-fixation method for minute metazoans.  Nematology 19, 871-881 [Abstract]. [oral presentation].

Yushin, V.V., Claeys, M., Slos, D. and Bert, W. Male Gametes in nematodes: the case of the order Rhabditida (Nematoda).[abstract].[oral presentation by Yushin, V.V.].



9th International Nematology Symposium "Nematodes of natural and transformed ecosystems". Petrozavodsk, Russia, July 11– 5, 2011.


Yushin, V.V., Claeys, M.  Houthoofd, W. Mature spermatozoa of Brevibucca sp. (Nematoda: Rhabditida: Brevicuccidae). // Russian Journal of Nematology. 2011. 19: P. 182. [Abstract]. [Poster presented by Claeys M.].


Bert, W., V. V. Yushin, M. Couvreur, and M. Claeys. 2011. From the backbone of nematode phylogeny to species delineation: integrating morphology and sequences. Russian Journal of Nematology. 2011. 19: P. 172. [Abstract].


10th International Nematology Symposium. Moscow, Russia. June 30-July 5, 2013.

Claeys, M., V. V. Yushin, and W. Bert. 2013. High pressure freezing (HPF) and automatic freeze substitution (AFS): innovative techniques for transmission electron microscopy (TEM) in nematology. Russian Journal of Nematology. 2013. Vol. 18, № 2. P. 143-144. [Abstract]. [Poster presented by Claeys M.].


Yushin V.V., Claeys M., Bert W. Major sperm protein (MSP) in nematode sperm // Russian Journal of Nematology. 2013. Vol. 18, № 2. P. 162-163. [Abstract].


Bert, W., T. Janssen, D. Slos, A. Y. Kolombia, M. Claeys, M. Couvreur, C. Mermans, P. Ensafi, J. S. Quisado, Q. Xue, G. Karssen, V. V. Yushin, and W. Decraemer.  Nematode morphology, taxonomy and phylogeny at Ghent University: a diversity of integrated toolkits. Russian Journal of Nematology. 2013. Vol. 18, № 2. P. 142. [Abstract].


Slos, D., P. Ensafi, M. Claeys, V. V. Yushin, and W. Bert. 2013. Ultrastructure of sperm development in the peanut-parasitic nematode Ditylenchus n. sp. Russian Journal of Nematology. 2013. Vol. 18, № 2. P. 160. [Abstract].


6th International Congress of Nematology, Capetown, South Africa, 2014.


Slos D., Ensafi P., Claeys M., Yushin V.V., Decraemer W., Bert W. Ultrastructure of sperm development in the genus Ditylenchus // Journal of Nematology. 2014. Vol. 46. № 2. P. 237. [Abstract].


11th International Nematology Symposium «The nematodes and other Ecdysozoa», Cheboksary, Chuvash Republic, Russian Federation, 2015.


Limantseva L.A., Yushin, V.V., Bert, W., Claeys, M., Slos, D. Spermatogenesis and sperm ultrastructure of Anaplectus porosus Allen & Nofsinger, 1968 (Nematoda: Plectida) // Russian Journal of Nematology. V. 23. P. 142.