Burkholderia arrays and protocols

 

As the CFFT funded Burkholderia array program has now finished, Dr. Mahenthiralingam & co-workers have prepared a complete set of protocols for array analysis (GeneExpressionAnalysis Protocol_Cardiff_V4) for your information.

If you would like to perform further analysis using the current B. cenocepacia J2315 array then it can be purchased from Agilent (order number amidid#017397).

We also completed the design of six new arrays for the completed Burkholderia genomes (www.burkholderia.com): Burkholderia cenocepacia HI2424, Burkholderia cenocepacia AU1054,
Burkholderia cepacia AMMD (B. ambifaria AMMD), Burkholderia multivorans ATCC 17616,
Burkholderia vietnamiensis G4, and Burkholderia sp. 383 (Burkholderia lata 383).

 

These are available for purchase from OGT who then have them printed by Agilent in exactly the
same format as the current J2315 array. The cost is around 596 per slide (149 per array).
The contact for sales at OGT is:

Rachel Harper
Sales Executive
Oxford Gene Technology
Begbroke Science Park
Sandy Lane, Yarnton
Oxford OX5 1PF UK

T: +44 (0)1865 856 830
F: +44 (0)1865 848 684
E: rachel.harper@ogt.co.uk
W:
www.ogt.co.uk

The team of dr. Mahenthiralingam has used the B. ambifaria AMMD and B. lata 383 array experimentally and found their performance to be excellent using the protocols shown below and running genomic DNA as a reference channel.

If you need any further information on using arrays in future please feel free to get in touch with : .

Eshwar Mahenthiralingam, Ph.D,
Reader in Molecular Microbiology,
Cardiff School of Biosciences,
Cardiff University, Main Building Room 0.11E,  

Museum Avenue, Cardiff, Wales,

United Kingdom, CF10 3AT
Tel. +44 (0)29 20875875
Mobile 07850 949521
Fax. +44 (0)29 20874305
email:  MahenthiralingamE@cardiff.ac.uk

 

 

Documents (in alphabetical order, all in pdf format)

Bioanalyzer_RNA6000Nano_Assay

CyScribe_GFX_Purification_Kit

CyScribe_Post-Labelling_GE_Healthcare

GeneExpressionAnalysisProtocol_Cardiff_V4

Genomic_DNA_labeling_GEHealthcare

Hybridization_TwoColourProtocol_v5.5_Agilent

OGT_New_Burkholderia_Arrays

RiboPure_Bacteria_Ambion