(last update : 20-05-2012)

Next Generation Sequencing


This is a presentation I have made : Next Generation Sequencing for Dummies (download file). Explanation is available in this file.

It is a presentation of 128 slides, and still it is only the 'tip of the iceberg' when it goes about Next Generation Sequencing. With this presentation, I'm trying to give you the basic principles and work flow, so if you know what is happening, you can understand what can go wrong. Once you know the basics, it is easier to read more detailed publications.


I have made several pictures for this presentation. I have placed them here, available for others to use them. (Right-click to view or save the picture in full size)


Emulsion PCR explaned in detail.


Library preparation for Next Generation Sequencing.

Particle enrichment with magnetic beads.


Sequencing the DNA on the beads.


SOLiD ligation. This is just the first picture. All the pictures are available in this ZIP-file.


Mate-pair sequencing.


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